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rt 2 first strand kit  (Qiagen)


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    Structured Review

    Qiagen rt 2 first strand kit
    Rt 2 First Strand Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+2+first+strand+kit/rt2+first+strand+kit/pmc12273472-69-21-26
    Average 90 stars, based on 1 article reviews
    rt 2 first strand kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Molecular Crosstalk Between SIRT1, Wnt/β-Catenin Signaling, and Inflammatory Pathways in Renal Transplant Rejection: Role of miRNAs, lncRNAs, IL-1, IL-6, and Tubulointerstitial Inflammation
    Article Snippet: .. For cDNA synthesis, the QIAGEN RT 2 First Strand Kit was used. .. In this stage, a 4 μL RNA sample was mixed with 1 μL of GE buffer and incubated at 42 °C for 5 min. Then, 2 μL of 5× BC3 buffer, 0.5 μL of Control P2, 1 μL of reverse transcriptase enzyme, and 1.5 μL of RNase-free water were added to initiate the cDNA synthesis reaction.

    Article Title: BRCA1 influences whole-body metabolism in humanized mice
    Article Snippet: A small amount of muscle tissue was removed from the hindlimb of 4 wild-type and 4 humanized BRCA1 female mice and RNA was extracted using the RNeasy Mini Kit (Qiagen). .. RNA quality was assessed using the NanoDrop Eight Spectrophotometer and 1μg total RNA was used in the cDNA synthesis using the RT 2 First Strand Kit (Qiagen). .. RT 2 Profiler PCR Array Mouse Glucose Metabolism (Geneglobe ID PAMM-006Z) was performed for each of the cDNA samples using the Qiagen protocol for ABI Step One Plus.

    Article Title: HCV-related hepatocellular carcinoma: gene signatures associated with TERT promoter mutations and sex
    Article Snippet: .. The RT 2 First Strand kit (Qiagen) was used for cDNA synthesis, according to the manufacturer’s instructions. ..

    Spectrophotometry:

    Article Title: BRCA1 influences whole-body metabolism in humanized mice
    Article Snippet: A small amount of muscle tissue was removed from the hindlimb of 4 wild-type and 4 humanized BRCA1 female mice and RNA was extracted using the RNeasy Mini Kit (Qiagen). .. RNA quality was assessed using the NanoDrop Eight Spectrophotometer and 1μg total RNA was used in the cDNA synthesis using the RT 2 First Strand Kit (Qiagen). .. RT 2 Profiler PCR Array Mouse Glucose Metabolism (Geneglobe ID PAMM-006Z) was performed for each of the cDNA samples using the Qiagen protocol for ABI Step One Plus.

    Isolation:

    Article Title: Bioengineering of novel organotypic 3D human liver tissue model for drug-induced liver injury and toxicity studies
    Article Snippet: .. Total RNA (500 ng) isolated from 3D liver tissues was reverse transcribed using the RT 2 First Strand Kit (Qiagen Cat# 330404) following the manufacturer’s recommendations. .. The cDNA synthesis was performed on an Eppendorf Mastercycler Gradient (Eppendorf, Enfield CT) by incubating samples with reverse transcriptase enzyme at 42°C for 15 min, followed denaturation by incubating the cDNA at 95°C for 5 min.

    Reverse Transcription:

    Article Title: Bioengineering of novel organotypic 3D human liver tissue model for drug-induced liver injury and toxicity studies
    Article Snippet: .. Total RNA (500 ng) isolated from 3D liver tissues was reverse transcribed using the RT 2 First Strand Kit (Qiagen Cat# 330404) following the manufacturer’s recommendations. .. The cDNA synthesis was performed on an Eppendorf Mastercycler Gradient (Eppendorf, Enfield CT) by incubating samples with reverse transcriptase enzyme at 42°C for 15 min, followed denaturation by incubating the cDNA at 95°C for 5 min.

    Article Title: Evidence for a JAK2/STAT3 proinflammatory and vasculogenic mimicry interrelated molecular signature in adipocyte-derived mesenchymal stromal/stem cells
    Article Snippet: Premade RT 2 Profiler PCR arrays for Human Inflammatory Cytokines and Receptors (PAHS-011ZD) were purchased from QIAGEN and used following the manufacturer’s instructions. .. Briefly, the genomic DNA was removed before 0.5 μg of total RNA was reverse transcribed via the RT 2 First Strand Kit (QIAGEN, 330404). .. Each plate was used to assess one cDNA sample prepared with RT 2 SYBR Green qPCR Mastermix (QIAGEN, 330502).

    Purification:

    Article Title: Inhibition of microglia priming by NLRP3 reduces the impact of early life stress and mild TBI
    Article Snippet: .. RNA was extracted from the tissue with a TRIzolTM plus RNA purification kit (Thermo Fisher Scientific). cDNA was synthesized using the RT 2 First strand kit (Qiagen). .. Quantitative, real-time PCR was performed with RT 2 qPCR primer assays for Hmgb1 , Tlr4 , Nlrp3 , Casp1 , Il1b , Nr3cl , and Gapdh and RT 2 SYBR Green Mastermix (Qiagen) using an Applied Biosystems 7300r real-time PCR System (Thermo Fisher Scientific).

    Synthesized:

    Article Title: Inhibition of microglia priming by NLRP3 reduces the impact of early life stress and mild TBI
    Article Snippet: .. RNA was extracted from the tissue with a TRIzolTM plus RNA purification kit (Thermo Fisher Scientific). cDNA was synthesized using the RT 2 First strand kit (Qiagen). .. Quantitative, real-time PCR was performed with RT 2 qPCR primer assays for Hmgb1 , Tlr4 , Nlrp3 , Casp1 , Il1b , Nr3cl , and Gapdh and RT 2 SYBR Green Mastermix (Qiagen) using an Applied Biosystems 7300r real-time PCR System (Thermo Fisher Scientific).

    Article Title: Blockade of IgG Fc receptors reduces pain after intervertebral disc injury
    Article Snippet: Total RNA was extracted by using the RNeasy Mini Kit (Cat# 74104, Qiagen), and the purity and concentration were determined spectrophotometrically. .. Complementary DNA was synthesized from 1 μg RNA using RT 2 First Strand Kit (Cat# 330404, Qiagen). .. Real-time polymerase chain reactions were performed on the QuantStudio 7 Flex Real-Time PCR system (Thermo-Fisher Scientific) using RT 2 qPCR Primer Assay and the RT 2 SYBR Green qPCR Mastermix (Cat# 330523, Qiagen).18 S Expression was used as an internal reference.

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: Total RNA was isolated using RNeasy Mini kit (74104, Qiagen). .. The cDNA was synthesized using RT 2 First Strand Kit (330404, Qiagen). qRT-PCR was performed using RT 2 SYBR Green qPCR Mastermix (330502, Qiagen) with an ABI Step OnePlus Real-Time PCR system (Applied Biosystems) according to the operator’s manual. ..

    Quantitative RT-PCR:

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: Total RNA was isolated using RNeasy Mini kit (74104, Qiagen). .. The cDNA was synthesized using RT 2 First Strand Kit (330404, Qiagen). qRT-PCR was performed using RT 2 SYBR Green qPCR Mastermix (330502, Qiagen) with an ABI Step OnePlus Real-Time PCR system (Applied Biosystems) according to the operator’s manual. ..

    SYBR Green Assay:

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: Total RNA was isolated using RNeasy Mini kit (74104, Qiagen). .. The cDNA was synthesized using RT 2 First Strand Kit (330404, Qiagen). qRT-PCR was performed using RT 2 SYBR Green qPCR Mastermix (330502, Qiagen) with an ABI Step OnePlus Real-Time PCR system (Applied Biosystems) according to the operator’s manual. ..

    Real-time Polymerase Chain Reaction:

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: Total RNA was isolated using RNeasy Mini kit (74104, Qiagen). .. The cDNA was synthesized using RT 2 First Strand Kit (330404, Qiagen). qRT-PCR was performed using RT 2 SYBR Green qPCR Mastermix (330502, Qiagen) with an ABI Step OnePlus Real-Time PCR system (Applied Biosystems) according to the operator’s manual. ..



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